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ZOOMplan
Onion Skins
(ZOOMplan developed by Dr. Vickie Harry, Clarion, Pennsylvania)
What structures can be observed in onion cells?

The life activities of a cell take place within the cytoplasm. Most plant cells have similar intercellular organization and most cellular activities are somewhat similar. The nucleus is the center of control and regulates cell processes. The DNA, responsible for passing on features from one generation to another is found in the nucleus. Other structures of the cell include the cell wall (support and protection), cell membrane (allows dissolved materials to enter and leave the cell), vacuole (storage for food and waste), cytoplasm (where other important cell structures are found), and chloroplasts (green colored bodies which contain chlorophyll which the plant uses to make food).

Science: Observational investigation of the structures of onion cells

Objective: Using DigiScope technology, MAIS elementary and middle school students will observe an onion cell’s nucleus, cytoplasm, cell wall, and cell membrane.

Concepts: Structure and function of cellular organization in plants

Processes: Observing, describing, identifying, predicting, measuring, collecting data, recording data, displaying and interpreting data

Project AERO Standards: Science: # 1,5, a. Keep records of investigations and observations and not alter the records. Science: # 2, 5, c. Know how to make sketches or models to aid in explaining scientific procedures or ideas. Science: # 4, 5, a. Use technology, including cameras, tape recorders, and computers, to store and retrieve verbal and graphic information and data. b. Use a variety of scientific tools to collect data. Science: # 16, 5, a. Identify the cell as the fundamental unit of living organisms and recognize that some organisms are unicellular, while others are multicellular. Technology: # 1, 5, a. Use keyboards and other common input and output devices. b. Discuss common uses of technology in daily life and the advantages and disadvantages those uses provide. Math: # 2, 3, c. Explain why a prediction, estimation, or solution is reasonable. Math: # 7, 3, a. Estimate before measuring to determine the reasonableness of a solution. b. Estimate and measure length, time, temperature, and weight to the nearest unit using customary, metric, and nonstandard measurement. Math: # 10, 3, a. Pose a question and collect data by observing, measuring, surveying, and counting. English: # 9, 4, b. Gather and record information (e.g., note taking, photography, surveys). d. Use standard reference tools (e.g., dictionary, thesaurus, library information systems, encyclopedia, on-line information) to gather information for research. Social Studies: # 8, 5, b. Examine ways in which tools and techniques make certain tasks easier. d. Describe instances in which changes in values, beliefs, and attitudes have resulted from new scientific knowledge and from technological knowledge.

Materials: Raw onion, knife, forceps, slides and cover slips, Methylene Blue stain solution, droppers and water, paper towels, DigiScopes

Procedure:
1. After cutting the onion, pull it apart in sections. Using the forceps, pull off a piece of the thin membrane (skin).
2. Place the skin in the center of the slide and add a drop of water.
3. Cover the skin with a cover slip, pressing down to remove air bubbles.
4. View the onion skin at different magnifications to observe the detail of cellular structure.
5. Remove the eyepiece from the DigiScope and insert the camera in its place. Now go to the main menu of Motic Play and click the Capture button. Print the image by clicking on the Jump to Edit button. When in the editing window, click the Print button. Examine the printed cells and label their parts.
6. Use a strand of your hair to measure the cells by placing a short piece of hair across the skin. Predict how many widths of your hair you could lay side by side over the length of one cell or the width of one cell.
7. Discuss Robert Hooke’s discovery of cells in 1655.
8. Remove this slide from the microscope and prepare another slide of onion skin. Stain this specimen with a drop of Methylene Blue. Repeat step 5.
9. Compare the images of the stained and unstained specimens. Identify the structures of the onion cell on the captured images. Students may want to make an illustrated booklet for a PowerPoint presentation of what they learned.

References:
AIMS Education Foundation. (1995). Magnificent Microworld Adventures. Fresno, CA: AIMS Education Foundation.
AIMS Education Foundation Newsletter. (1987). Important Things Come in Small Packages. Fresno, CA: AIMS Education Foundation.
K – 8 Experiment Manual. (2002). Observing the Onion Skin. Science Kit and Boreal Laboratories & Motic Incorporation Limited.

Dr. Ken Mechling • Project Director • 1305 Robinwood Drive • Clarion, Pennsylvania 16214 USA
Reina O'Hale • Executive Director, MAIS • Madrid, Spain
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