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ZOOMplan
Planarians
(ZOOMplan developed by Dr. Vickie Harry, Clarion, Pennsylvania)
What structures and behaviors can be observed in planarians?

Planarians belong to the Turbellaria, the only free-living class in the phylum Platyhelminthes (flatworms). The term planarian encompasses all triclad turbellarians. An intestine that has three branches distinguishes the triclads. The brown planarian, Dugesia tigrina, has pigmented eye spots near the anterior end giving it a cross-eyed appearance. Planarians can regenerate significant portions of the body if cut into two or more pieces. Planarians move by gliding on the mucus they secrete. They prey on animals that become entangled in their mucus trail. Planarians are found in brooks, streams, ponds, and lakes. Planarians may be purchased through a science supply house or captured in the field. One method for collecting planarians is to place small chunks of fresh, raw liver on the bottom of a stream for pond near shore for a few hours. After retrieving the liver, flush specimens into a dish with a stream of water from a water bottle.

Planarians have participated in NASA experiments in space to determine whether or not they would regenerate in space as they do on Earth. There is interest in whether planarians regenerate faster in space and if zero gravity affects their regrowth.

Science: Observational investigation of the structures and behaviors of planarians

Objective: Using DigiScope technology, MAIS elementary and middle school students will observe and describe the structure and movements of planarians.

Concepts: Structure and movements of flatworms

Processes: Observing, describing, communicating, identifying, collecting data, recording data, and investigating organisms that live in a drop of pond water.

Project AERO Standards: Science: # 4 Scientific Inquiry: Students will be able to select and use tools and instruments to conduct scientific investigations. Science: # 1,5, a. Keep records of investigations and observations and not alter the records. Science: # 2, 5, c. Know how to make sketches or models to aid in explaining scientific procedures or ideas. Science: # 3, 5, d. Explain why scientists use technology in investigations, including to increase their power of observation and to measure and compare accurately. Science # 15, 8, d. Describe responses of plants and animals to various stimuli in their environment. Science: # 4, 5, a. Use technology, including cameras, tape recorders, and computers, to store and retrieve verbal and graphic information and data. b. Use a variety of scientific tools to collect data. Technology # 3 Students will gather, analyze, interpret, synthesize, apply and communicate information and designs using technology tools. Technology: # 3, 5, d. Determine when technology is useful and select the appropriate tool(s) and technology resources to address a variety of tasks and problems.

Materials: Planarians, well slides and coverslips, droppers and spring water, paper towels, DigiScopes

Procedure:
1. Secure planarians.
2. Maintain planarians in spring water or clean water from a pond or stream. Do not use tap water since chlorine and other additives may be toxic to the worms.
3. Place a planarian in spring water in a well slide and cover with a coverslip.
4. View the planarians at different magnifications to observe the detail of structure and movement.
5. Remove the eyepiece from the DigiScope and insert the camera in its place. Now go to the main menu of Motic Play and click the Capture button. Print the image by clicking on the Jump to Edit button. When in the editing window, click the Print button.
6. Find the anterior end of the planarian. Describe the eyespots.
7. Describe the movements of the planarians.
8. You can create a video of planarian movement for a specific time limit by clicking on the time setting button on the Capture menu. To set the time limit, click on the 15 button on the panel or click and move the slider to 15 seconds. Next, click on the Record Video button on the Capture Menu. The Name File box will appear at the top of the image window. After you type in the name for the video, click and drag the Name File box to the side so the image can be seen. When you are ready to begin the video of the planarian’s movements, click Open and quickly click OK to begin recording. After 15 seconds, the video will automatically stop. Replay the video for multiple viewings and data recording. Additional videos can be recorded to research specific investigations.
9. Design an investigation to inquire about planarians.
10. Identify the structures and movements of the planarians on the captured images. Students may want to make an illustrated booklet for a PowerPoint presentation of what they investigated.

References:
Amery, H. & Songi, J. (1994). Discover Hidden Worlds The Home. New York: A Golden Book.
Marcus, B. A. (1988). Carolina Tips Planarians. Burlington, North Carolina: Carolina Biological Supply Company.
Snedden, R. (1996). Yuck! A Big Book of Little Horrors. New York, New York: Simon and Schuster.
Usborne Science and Experiments. (1989). The World of the Microscope. London, England: Usborne Publishing, Ltd.

Dr. Ken Mechling • Project Director • 1305 Robinwood Drive • Clarion, Pennsylvania 16214 USA
Reina O'Hale • Executive Director, MAIS • Madrid, Spain
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