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ZOOMplan
You...Instead of the Onion Skin
(ZOOMplan developed by Dr. Vickie Harry, Clarion, Pennsylvania)
What structures can be observed in epithelial cells?

The epithelial cells, which form the outer layer of skin of animals and also line certain body cavities, are flattened pancake-like cells, arranged in sheets with no spaces between them. New cells are constantly being formed under the layer, this replacing the surface cells that are worn off. Cells performing the same function often are similar in shape. Epithelial cells, whether from fish, frog, bird, or human, look somewhat alike. The outer skin of frogs, salamanders, or other animals can be examined under the microscope. Small pieces of cast-off skin can be found floating in an aquarium where aquatic animals are kept. There are similarities and differences between plant and animal cells. Onion cells and human cells differ in size and shape. The onion cells are thicker than the flat animal skin cells. Both types of cells have a nucleus. The onion cells have thick, nonliving cell walls (as do most plant cells); the animal cells do not. Cell membranes are the living outer boundaries of plant and animal cells. In plants, living membranes are usually closely surrounded by cell walls and cannot be distinguished easily under a microscope.

Science: Observational investigation of the structure of human epithelial cells

Objective: Using DigiScope technology, MAIS elementary and middle school students will observe their own epithelial cells from the inside linings of their cheeks.

Concepts: Structure and function of epithelial cellular organization in animals

Processes: Observing, describing, identifying, communicating, predicting, measuring, collecting data, recording data, displaying and interpreting data, comparing data

Project AERO Standards: Science: # 1,5, a. Keep records of investigations and observations and not alter the records. Science: # 2, 5, c. Know how to make sketches or models to aid in explaining scientific procedures or ideas. Science: # 16, 5, a. Identify the cell as the fundamental unit of living organisms and recognize that some organisms are unicellular, while others are multicellular. Science: # 4, 5, a. Use technology, including cameras, tape recorders, and computers, to store and retrieve verbal and graphic information and data. b. Use a variety of scientific tools to collect data. Math: # 7, 3, a. Estimate before measuring to determine the reasonableness of a solution. b. Estimate and measure length, time, temperature, and weight to the nearest unit using customary, metric, and nonstandard measurement.

Materials: Flat wooden toothpicks, epithelial cells from cheek linings, slides and coverslips, Methylene Blue stain solution, droppers and water, paper towels, DigiScopes

Procedure:
1. Using a dropper, place two drops of water on a slide.
2. Collect human epithelial cells by gently scraping the inside of your cheek with the wide end of a flat toothpick. Touch the material collected on the toothpick to the water on the slide.
3. Add a drop of Methylene Blue and cover with a coverslip.
4. View the epithelial cells at different magnifications to observe the detail of cellular structure.
5. Remove the eyepiece from the DigiScope and insert the camera in its place. Now go to the main menu of Motic Play and click the Capture button. Print the image by clicking on the Jump to Edit button. When in the editing window, click the Print button.
6. Use a strand of your hair to measure the cells by placing a short piece of hair across the epithelial cells. Predict how many widths of your hair you could lay side by side over the length of one cell or the width of one cell.
7. Compare the images of the plant cells from an onion skin to the human epithelial cells. Compare the similarities and the differences.
8. Locate and label the parts of the plant cells and the animal (cheek) cells.
9. Prepare a slide with both onion cells and epithelial cells on it. Students may want to make an illustrated booklet for a PowerPoint presentation comparing the plant and animals cells.

References:
AIMS Education Foundation. (1995). Magnificent Microworld Adventures. Fresno, CA: AIMS Education Foundation.
K – 8 Experiment Manual. (2002). Dancing Cheek to Cheek. Science Kit and Boreal Laboratories & Motic Incorporation Limited.

Dr. Ken Mechling • Project Director • 1305 Robinwood Drive • Clarion, Pennsylvania 16214 USA
Reina O'Hale • Executive Director, MAIS • Madrid, Spain
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